RESUMO
In Brazil, rabies occurs mainly within an urban cycle, in which dogs and bats are reservoirs. This paper aims to report the occurrence of rabies in Callithrix sp. in Niterói, Rio de Janeiro, Brazil. In June 2019 a hybrid specimen was referred for diagnosis. The Direct Fluorescent Antibody, Mouse Inoculation, and Polymerase Chain Reaction tests were positive. A phylogenetic analysis was compatible with antigenic variant 3, characteristic of Desmodus rotundus. New studies should be undertaken to elucidate the real role of callitrichids in the urban rabies cycle.
Assuntos
Callithrix/virologia , Vírus da Raiva/genética , Raiva/diagnóstico , Animais , Brasil , Técnica Direta de Fluorescência para Anticorpo , Filogenia , Reação em Cadeia da Polimerase , Vírus da Raiva/imunologia , População UrbanaRESUMO
Abstract In Brazil, rabies occurs mainly within an urban cycle, in which dogs and bats are reservoirs. This paper aims to report the occurrence of rabies in Callithrix sp. in Niterói, Rio de Janeiro, Brazil. In June 2019 a hybrid specimen was referred for diagnosis. The Direct Fluorescent Antibody, Mouse Inoculation, and Polymerase Chain Reaction tests were positive. A phylogenetic analysis was compatible with antigenic variant 3, characteristic of Desmodus rotundus. New studies should be undertaken to elucidate the real role of callitrichids in the urban rabies cycle.
Assuntos
Animais , Raiva/diagnóstico , Vírus da Raiva/genética , Callithrix/virologia , Filogenia , Vírus da Raiva/imunologia , População Urbana , Brasil , Reação em Cadeia da Polimerase , Técnica Direta de Fluorescência para AnticorpoRESUMO
An epidemic-geographic rabies study was carried out in which 72 animal and human brain samples were analyzed for Lyssaviruses by a direct immunofluorescent technique (DIFT) and a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Fifty-two samples were also tested by a mouse inoculation test. Lyssavirus RNA was detected in 60 of 72 samples. Five DIFT-negative bat samples tested by a nested PCR assay showed evidence of the presence of rabies virus RNA. Sequencing of amplified rabies virus nucleoprotein encoding segments of a selection of the samples resulted in the formation of clusters, corresponding to samples originating from cattle and equines from the same hydrographic basin. Genomically related Lyssavirus strains of bat origin were found in each cluster, most likely because of the role of the bat in the epidemiology of the virus. All samples studied were of genotype 1. With exception of the human sample, all were distinct from the reference sample.
